DOI: 10.1055/a-2165-1000 ISSN:

Anti-thrombotic effects mediated by a novel dual-target peptide inhibiting both platelet aggregation and thrombin activity without causing bleeding

Jinchao Yu, Tianfa Wang, XiaoHan Zhang, Qing Chen, Yajun Hu, Qiaoyan Liang, Yun Shi, Yuxiong Wang, Yanling Zhang, Min Yu, Bing Zhao
  • Hematology

Classical anticoagulants and antiplatelets are associated with high frequencies of bleeding complications or treatment failure when used as single agents. Thrombin plays an important role in the blood coagulation system. GP IIb/IIIa is the central receptor of platelets, which can recognize the Arg-Gly-Asp (RGD) sequence and activate platelets. We have constructed a novel dual-target peptide (PTIP) based on the direct thrombin inhibitor peptide (DTIP) via molecular simulation and homology modeling. PTIP was expressed at high levels in Pichia pastoris. PTIP interfered with thrombin-mediated coagulation and ADP-induced platelet aggregation in vitro. When injected intravenously or subcutaneously, PTIP showed potent and dose-dependent extension of aPTT and PT which were similar to DTIP; but only PTIP was capable of inhibiting platelet aggregation. The antithrombotic activity of PTIP was determined by ferric chloride injury model, pulmonary thromboembolism model, and arteriovenous bypass thrombosis model. PTIP (1.0 mg/kg) decelerated thrombosis formation in venous and arterial vessels induced by FeCl3 injury. PTIP (1.0 mg/kg) also prevented deep venous thrombosis and increased the survival rate associated with pulmonary thromboembolism. And PTIP effectively reduced thrombus length in arteriovenous bypass thrombosis model. Moreover, the antithrombotic dose of PTIP could not induce bleeding determined by transecting distal tail assay. These data establish that PTIP represents a novel antithrombotic agent whose effects involve both inhibition of platelet activation and reduction of fibrin generation. And PTIP not only can be used in venous thrombosis and arterial thrombosis, it can also replace the combined treatment of antiplatelet and anticoagulant drugs in thrombotic diseases.

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