DOI: 10.2478/rtuect-2024-0002 ISSN: 2255-8837

Supercritical CO2 Extraction of Fish Roe

Ilze Luksta, Taras Mika, Kriss Spalvins
  • General Environmental Science
  • Renewable Energy, Sustainability and the Environment


Seafood is rich in omega-3 polyunsaturated fatty acids, and the good emulsifying properties of phospholipids make them suitable for use in the food, pharmaceutical and cosmetic industries. This study attempted to evaluate different fish species obtained using supercritical carbon dioxide without and with and ethanol as co-solvent extraction and organic solvent (ethanol) extraction. Supercritical CO2 extraction was performed at 30–50 °C and at different pressures (17.7–33.0 MPa), maintaining a CO2 flow rate of 0.6 mL/min for 3 hours. Gas chromatography and Bligh & Dyer methods were used to analyze the extracts. The results showed that only one species of fish roe (rainbow trout) produced oil, but it was concluded that improvements were needed to be able to extract oil from African catfish roe using supercritical CO2 extraction. Rainbow trout roe contains the most monounsaturated fatty acids (44 %).

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