YTnC2 , an improved genetically‐encoded green calcium indicator based on toadfish troponin C
Oksana M. Subach, Anna V. Vlaskina, Yulia K. Agapova, Alena Y. Nikolaeva, Anna M. Varizhuk, Oleg V. Podgorny, Kiryl D. Piatkevich, Maxim V. Patrushev, Konstantin M. Boyko, Fedor V. Subach - General Biochemistry, Genetics and Molecular Biology
Genetically‐encoded calcium indicators (GECIs) based on truncated troponin C are attractive probes for calcium imaging due to their relatively small molecular size and two‐fold reduced calcium ion buffering. However, the best‐suited members of this family, YTnC and cNTnC, suffer from low molecular brightness, limited dynamic range, and/or poor sensitivity to calcium transients in neurons. To overcome these limitations, we developed an enhanced version of YTnC, named YTnC2. Compared to YTnC, YTnC2 had 5.7‐fold higher molecular brightness and 6.4‐fold increased dynamic range in vitro. YTnC2 was successfully used to reveal calcium transients in the cytosol and in the lumen of mitochondria of both mammalian cells and cultured neurons. Finally, we obtained and analyzed the crystal structure of the fluorescent domain of the YTnC2 mutant.