Quantification and Analytical Validation of Atomoxetine by Liquid Chromatographic Method through Isocratic Separation in Pharmaceutical Dosage Forms

The quantification and analytical validation of Atomoxetine was developed with a stationary phase Phenomenex column C18 (4.6mm×250mm, 5µm) and LC1220 Agilent HPLC instrument equipped with variable wavelength detector at 270nm and Acetonitrile: Water containing 0.2% of triethylamine (85:15, v/v) was used as mobile phase passed at a flow rate 1mL/min. Elution takes place at retention time of 2.283min. Validation of method were performed as per ICH guidelines which shows linearity concentration range from 10-125µg/mL amid Correlation coefficient=0.999 with Y = 63164x+64858 regression equation obtained. LOD and LOQ of Atomoxetine were 0.07µg/mL and 0.21µg/mL correspondingly. Recovery of Atomoxetine was obtained 99.83-100.14%.