DOI: 10.4155/bio-2023-0132 ISSN:

Influence of preanalytical and analytical factors on the quantification of six regulatory serum proteins

Felix Menne, Nicolas A Henzen, Marc Sollberger, Andreas U Monsch, Carola G Schipke
  • Medical Laboratory Technology
  • Clinical Biochemistry
  • General Pharmacology, Toxicology and Pharmaceutics
  • General Medicine
  • Analytical Chemistry

Background: We analyzed differences in protein concentrations in human blood serum depending on the tube material and the immunoassay platform used. Materials & methods: Blood samples from study participants were collected in glass and polypropylene tubes (n = 292). Serum concentrations of six proteins (BDNF, IGF-1, VEGF-A, TGF-β1, MCP-1 and IL-18) were assessed by using ELISAs (all biomarkers), as well as a novel fully automated immunoassay platform (all but IGF-1, n = 211). Bland–Altman analyses were conducted to investigate intrasample variability of protein concentrations. Results: Tube comparison resulted in mean biases of between -0.45 and -70.64%. Platform comparison revealed mean biases of between 21.04 and -128.10%. Conclusion: Protein concentrations can vary significantly depending on the types of tube and immunoassay used, with protein-specific differences.

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