DOI: 10.3390/mps6050077 ISSN:
Improvement of Targeted Fungi Secondary Metabolite Production Using a Systematic Experimental Design and Chemometrics Analysis
Lamonielli F. Michaliski, Laura P. Ióca, Leandro S. Oliveira, Camila M. Crnkovic, Mirelle Takaki, Vitor F. Freire, Roberto G. S. Berlinck- Biochemistry, Genetics and Molecular Biology (miscellaneous)
- Structural Biology
- Biotechnology
Fungi are well-known producers of chemically diverse and biologically active secondary metabolites. However, their production yields through fermentation may hamper structural analysis and biological activity downstream investigations. Herein, a systematic experimental design that varies multiple cultivation parameters, followed by chemometrics analysis on HPLC-UV-MS or UHPLC-HRMS/MS data, is presented to enhance the production yield of fungal natural products. The overall procedure typically requires 3–4 months of work when first developed, and up to 3 months as a routine procedure.