DOI: 10.4103/jcde.jcde_244_23 ISSN: 2950-4716

Dentinal tubule penetration following ultrasonic, sonic, and single-cone technique of a biosealer: An ex vivo study

Dina Abdellatif, Massimo Pisano, Renato Gullà, Giuseppe Sangiovanni, Shishir Singh, Francesco Giordano, Alessio Buonavoglia, Alfredo Iandolo



The endodontic space is complex, and using a single-cone obturation technique fails to ensure a complete filling.


This study aimed to investigate the effect of ultrasonic activation, sonic activation, and single-cone technique of a biosealer on its dentinal tubular penetration.

Materials and Methods:

In the experiment, single-root mandibular premolars were randomly assigned to three groups (n = 20): group A, ultrasonic activation; Group B, sonic activation; and Group C, single-cone technique. Penetration of the fluorescently labeled biosealer was investigated using a confocal laser scanning microscope.


The data were statistically analyzed using Kruskal–Wallis and Mann–Whitney tests (P = 0.05). The highest penetration of biosealer was observed in Group A, followed by Group B (P < 0.05).


Dentinal tubule penetration of biosealer was significantly improved by ultrasonic and sonic activation techniques.

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