Anticancer and antimicrobial potential of zinc/sodium alginate/polyethylene glycol/d-pinitol nanocomposites against osteosarcoma MG-63 cells
Anandaraj Lakshmanan, Chandramohan Govindasamy, Allur Subramaniyan Sivakumar, Samer Hasan Hussein-Al-Ali, Monishsanthosh Ramesh, Hariprasath Lakshmanan- Health, Toxicology and Mutagenesis
- Industrial and Manufacturing Engineering
- Fuel Technology
- Renewable Energy, Sustainability and the Environment
- General Chemical Engineering
- Environmental Chemistry
Abstract
Background
The field of nanomedicine has attracted much interest and is now serving as the impetus for many revolutionary advances in the pharmaceutical industry.
Objectives
In the current exploration, we intended to fabricate the zinc/sodium alginate/polyethylene glycol/
Methods
ZSP/D-Pin/NCs were synthesized and characterized using several techniques and their cytotoxicity was examined against osteosarcoma MG-63 cells and normal 3T3 cells using the MTT assay. The levels of oxidative stress and apoptotic protein were examined using assay kits and fluorescence staining.
Results and Conclusion
The findings of several characterization studies revealed the development of agglomerated and crystalline ZSP/D-Pin/NCs. The antimicrobial assay demonstrated that ZSP/D-Pin/NCs substantially inhibited the growth of pathogens. Additionally, the MG-63 cell viability, which was exposed to several doses (1–20 µg) of ZSP/D-Pin/NCs, showed a remarkable decrease at various time periods, i.e., 24, 48, and 72 h without showing toxicity in 3T3 cells. The results of the fluorescence staining assay demonstrated that ZSP/D-Pin/NCs considerably increased apoptosis in the MG-63 cells by triggering oxidative stress. The antioxidants were reduced and upregulated the Bax and caspase expressions in ZSP/D-Pin/NC-treated MG-63 cells.