Synthesis, characterization, and biological properties of novel Schiff bases containing pentafluorophenyl hydrazine

Abstract

In the present study, new Schiff bases derived from pentafluorophenyl‐hydrazine (L1, L2, L3, L4) were synthesized and their structures were characterized by ¹H nuclear magnetic resonance spectroscopy (NMR), ¹³C NMR, ¹⁹F NMR, fourier transform infrared spectroscopy, and elemental analysis methods. Then, the anticancer activities of the obtained compounds were investigated using three human cancer cell lines (A2780, over; Caco‐2, colon; and HT‐29 colon carcinoma cell lines). According to the obtained cytotoxicity results, compound number L4 was found to have the highest anticancer activity in A2780 (over) and Caco‐2 (colon) cell lines. Furthermore, in silico, ADMET properties, where studies play an important role in the development and prediction of drug compounds, were calculated using web‐based platforms. In addition, molecular docking studies were performed to evaluate the binding interactions between the synthesized pentafluorophenyl‐hydrazone compounds and the MDM2 protein (4JSC). Both in vitro and in silico results showed that the synthesized compounds could act as potent anticancer agents.