DOI: 10.1093/toxres/tfae017 ISSN: 2045-4538

Nigella sativa oil exhibits anti-aging effects in transgenic Alzheimer’s Drosophila melanogaster via anti oxidant pathways: survival-rate and life span studies

A I Mukhtar, B Danborno, A A Sadeeq
  • Health, Toxicology and Mutagenesis
  • Toxicology


In this article we aime to investigate he anti-aging effect of Nigella sativa oil. Transgenic flies engineered under UAS/GAL4 system were acquired from the Bloomington Drosophila stock center. For methodology, (LC50), 30 flies 2–3 days old were divided into four groups, and exposed to diets of 30, 60, 90, and 120 μL/10 g in 3 replicates at a density of 30 flies per vial. Mortality was recorded daily for 7 consecutive days. For survival, (150) 2–3 days adult flies were divided into four groups (I-IV), 100 flies in each group. Group I which served as control fed on 10 g of diet only, group II was fed 10 g of diet only, group III (AB42 + low dose) was fed on 10 g diet +6.39ul NSO, group IV (AB42 + high dose) was fed 10 diet + 12.77ul NSO. The administration lasted for 28 days. For the third phase, a similar protocol was adopted with each group having (400) flies, this phase lasted f till the last fly died. The effect of NSO was assessed by; Studying the mortality daily. Results revealed the lethal concentration of LC50 NSO to be 25.54 mg it was also observed from the study that exposure to NSO in food media at low doses has increased the lifespan of AB-42 treated flies. In conclusion, findings from this study suggest the efficacy of low-dose NSO increased the survival and life span of the AB-42 flies.

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