DOI: 10.2807/ ISSN: 1560-7917

Genomic characterisation of respiratory syncytial virus: a novel system for whole genome sequencing and full-length G and F gene sequences

María Iglesias-Caballero, Sara Camarero-Serrano, Sarai Varona, Vicente Mas, Cristina Calvo, María Luz García, Juan García-Costa, Sonia Vázquez-Morón, Sara Monzón, Albert Campoy, Isabel Cuesta, Francisco Pozo, Inmaculada Casas
  • Virology
  • Public Health, Environmental and Occupational Health
  • Epidemiology

To advance our understanding of respiratory syncytial virus (RSV) impact through genomic surveillance, we describe two PCR-based sequencing systems, (i) RSVAB-WGS for generic whole-genome sequencing and (ii) RSVAB-GF, which targets major viral antigens, G and F, and is used as a complement for challenging cases with low viral load. These methods monitor RSV genetic diversity to inform molecular epidemiology, vaccine effectiveness and treatment strategies, contributing also to the standardisation of surveillance in a new era of vaccines.

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