DOI: 10.1002/aic.18211 ISSN:

Engineering residues on C interface to improve thermostability of nitrilase for biosynthesis of Pregabalin precursor

Xia‐Feng Lu, Xiao‐Ling Tang, Chen‐Hui Xu, Hong‐Juan Diao, Zhe‐Ming Wu, Ren‐Chao Zheng, Yu‐Guo Zheng
  • General Chemical Engineering
  • Environmental Engineering
  • Biotechnology


Nitrilase‐mediated bioprocesses exhibited great potential in the production of value‐added carboxylic acids. However, poor thermostability of nitrilases usually restricts their industrial applications. Herein, the thermostability of nitrilase BaNITM0 was significantly improved by engineering the amino acid residues on the intersection of two dimers (C interface). Except for simultaneous enhancement of enantioselectivity and activity, the best variant V82L/M127I/L159M/F166Q/C237S/Q260H (BaNITM4) showed a 10.8‐fold increase in half‐life at 30°C compared with BaNITM0. Structural analysis demonstrated that additional hydrogen bonds were formed between the residues on the C interface, which strengthened the interactions of two symmetrical regions and spirals. Subsequently, the engineered nitrilase was immobilized onto epoxy resins LXTE‐603 and the immobilized nitrilase exhibited excellent stability over 12 repeated cycles, which indicated a great industrial potential for biosynthesis of Pregabalin precursor.

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