DOI: 10.1002/cpz1.1014 ISSN: 2691-1299

Data‐Independent Acquisition and Label‐Free Quantification for Quantitative Proteomics Analysis of Human Cerebrospinal Fluid

Karl A.T. Makepeace, Alexander W. Rookyard, Lipi Das, Badri N. Vardarajan, Jayanta K. Chakrabarty, Anu Jain, Min Suk Kang, Emily G. Werth, Dolly Reyes‐Dumeyer, Marielba Zerlin‐Esteves, Lawrence S. Honig, Richard Mayeux, Lewis M. Brown
  • Medical Laboratory Technology
  • Health Informatics
  • General Pharmacology, Toxicology and Pharmaceutics
  • General Immunology and Microbiology
  • General Biochemistry, Genetics and Molecular Biology
  • General Neuroscience
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Abstract

This article presents a practical guide to mass spectrometry–based data‐independent acquisition and label‐free quantification for proteomics analysis applied to cerebrospinal fluid, offering a robust and scalable approach to probing the proteomic composition of the central nervous system. © 2024 Wiley Periodicals LLC.

Basic Protocol 1: Cerebrospinal fluid sample collection and preparation for mass spectrometry analysis

Basic Protocol 2: Mass spectrometry sample analysis with data‐independent acquisition

Support Protocol: Data‐dependent mass spectrometry and spectral library construction

Basic Protocol 3: Analysis of mass spectrometry data

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