DOI: 10.1093/bbb/zbae030 ISSN: 1347-6947

Convenient pre-separation method depends on negative charges suitable for glycan sequencing analysis

Shigemitsu Segawa, Shunji Natsuka
  • Organic Chemistry
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • General Medicine
  • Biochemistry
  • Analytical Chemistry
  • Biotechnology
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Abstract

For precise structural analysis of complex glycans, it is necessary to fractionate glycans based on acidity prior to liquid chromatography-mass spectrometry (LC-MS) analysis. Conventional fractionation using high performance liquid chromatography (HPLC) is time consuming and not high throughput. Therefore, we developed a rapid method for the fractionation using a small cartridge column. This enables rapid multi-sample processing for glycan sequencing.

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