DOI: 10.1177/10225536231220839 ISSN: 1022-5536

An immunohistochemical and biomechanical evaluation of the effect of fat graft on tendon healing

Ahmet Oztermeli, Mehmet Fatih Okyay
  • Surgery


To investigate the efficacy of fat grafting in primary tendon healing through immunohistochemical and biomechanical examinations.

Material and Method

The study material comprised a total of 10 male Sprague-Dawley rats, each approximately 10 weeks old. All 10 rats were operated on bilaterally. The right Achilles tendon in all the animals was defined as the study group. The tendon was cut and then repaired, and then fat graft was applied to the repair area. The left Achilles tendon of all the rats constituted the control group. The tendon was cut and repaired with no further application. After 4 weeks, the rats were euthanised and samples were taken from the tendons for immunohistochemical and biomechanical examinations.


In the biomechanical evaluations, no statistically significant difference was determined between the groups in respect of peak load and stiffness values ( p: .068, p: .089, respectively). In the histopathological evaluation, the tenocyte value of the study group was superior to that of the control group ( p: .04). No statistically significant differences were determined between the groups in respect of the other histopathological parameters. In the immunohistochemical evaluations, the type I collagen and TGF values of the study group were found to be higher than those of the control group ( p: .011, p: .012, respectively).


Compared to stem cell applications, the use of fat grafting is clinically easy to apply, has low costs, and has been shown to contribute to tendon healing at an immunohistochemical level with increased collagen and TGF beta values.

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