An acetyltransferase effector conserved across Legionella species targets the eukaryotic eIF3 complex to modulate protein translation
Lukas Syriste, Deepak T. Patel, Peter J. Stogios, Tatiana Skarina, Dhruvin Patel, Alexei Savchenko- Virology
- Microbiology
By translocating effectors inside the eukaryotic host cell, bacteria can modulate host cellular processes in their favor. Legionella species, which includes the pneumonia-causing Legionella pneumophila, encode a widely diverse set of effectors with only a small subset that is conserved across this genus. Here, we demonstrate that one of these conserved effector families, represented by L. pneumophila VipF (Lpg0103), is a tandem Gcn5-related N-acetyltransferase interacting with the K subunit of human eukaryotic initiation factor 3 complex. VipF catalyzes the acetylation of lysine residues on the C-terminal tail of the K subunit, resulting in the suppression of eukaryotic translation initiation factor 3-mediated protein translation in vitro . These new data provide the first insight into the molecular function of this pathogenic factor family common across Legionellae .