DOI: 10.1093/lambio/ovad098 ISSN:

A novel Bacillus sp. with antagonistic activity against a plant pathogen, Fusarium graminearum, and its potential antagonistic mechanism

Shanrui Shen, Feifan Yu, Xinyi Hao, Jing Chen, Huan Gao, Xiaofang Lai
  • Applied Microbiology and Biotechnology

Abstract

Fusarium head blight is a wheat disease caused by plant pathogen Fusarium graminearum, which leads to crop yield losses and agricultural economic losses, as well as pose a threat to the environment and human health. Effective biocontrol of F. graminearum is urgent. An antagonistic strain HZ-5 with 59.2% antagonistic activity against F. graminearum in vitro had been isolated from sea mud of Haizhou Bay using dual-culture assay, which was highly homologous with Bacillus halosaccharovorans according to the 16S rRNA sequence. The antagonistic activity of HZ-5 had been further studied. HZ-5 had broad range antagonistic activity against another six plant pathogenic fungi and was effective in controlling Fusarium head blight of wheat in pot experiment. The substances with antagonistic activity were temperature insensitive, and had been purified by HPLC to prove to be secreted lipopeptides. The antagonistic substances induced the biosynthesis of chitin and glycerol, while Ergosterol (Erg), cholesterol (Cho) and phosphatidylcholine (PC) reduced their inhibitory effect on F. graminearum. These data would be helpful to provide a better biocontrol strain against Fusarium head blight, and to provide important basis to elucidate the antagonistic mechanism of biocontrol.

More from our Archive