A novel approach to enhance the performance of kallikrein 6 enzyme using Pichia pastoris GS115 as a host
Fatemeh Mahmoodi, Hamid Bakherad, Navid Mogharrab, Mohammad Rabbani- General Pharmacology, Toxicology and Pharmaceutics
Background and purpose:
Enzyme engineering is the process of raising enzyme efficiency and activity by altering amino acid sequences. Kallikrein 6 (KLK6) enzyme is a secreted serine protease involved in a variety of physiological and pathological activities. The increased expression of KLK6 plays a key role in various diseases. Instability and spontaneous activation and deactivation are major challenges in the study of this enzyme. This study aimed to create a stable pro-KLK6 enzyme by enzyme engineering, designing a specific cleavage site for enterokinase, and using
Experimental approach:
An engineered pro-KLK6 gene was cloned into the pPICZα A expression vector. Then, it was expressed in
Findings/Results:
The secretory form of the pro-KLK6 was expressed at about 11 mg/L in
Conclusion and implications:
A stable pro-KLK6 enzyme was produced using