DOI: 10.1002/cyto.b.22136 ISSN: 1552-4949

Use of a hybrid intelligence decision tree to identify mature B‐cell neoplasms

Inès Vergnolle, Theo Ceccomarini, Alban Canali, Jean‐Baptiste Rieu, François Vergez
  • Cell Biology
  • Histology
  • Pathology and Forensic Medicine



Mature B‐cell neoplasms are challenging to diagnose due to their heterogeneity and overlapping clinical and biological features. In this study, we present a new workflow strategy that leverages a large amount of flow cytometry data and an artificial intelligence approach to classify these neoplasms.


By combining mathematical tools, such as classification algorithms and regression tree (CART) models, with biological expertise, we have developed a decision tree that accurately identifies mature B‐cell neoplasms. This includes chronic lymphocytic leukemia (CLL), for which cytometry has been extensively used, as well as other non‐CLL subtypes.


The decision tree is easy to use and proposes a diagnosis and classification of mature B‐cell neoplasms to the users. It can identify the majority of CLL cases using just three markers: CD5, CD43, and CD200.


This approach has the potential to improve the accuracy and efficiency of mature B‐cell neoplasm diagnosis.

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