UiO‐66‐NH 2 and Magnetic Fe 3 O 4 @Cellu@UiO‐66‐NH 2
Siti Unvaresi Misonia Beladona, Meiyanti Ratna Kumalasari, Muhammad Irmawan, Retno AgnestisiaABSTRACT
This study investigated the synthesis, characterization, and performance of UiO‐66‐NH 2 and magnetic Fe 3 O 4 @Cellu@UiO‐66‐NH 2 composites as efficient supports for cellulase immobilization and reusability. UiO‐66‐NH 2 was synthesized using an amine‐functionalized ligand (H 2 BDC‐NH 2 ) to enhance its enzyme adsorption capacity, whereas Fe 3 O 4 nanoparticles were surface‐modified with cellulose to facilitate the growth of UiO‐66‐NH 2 crystals, forming a magnetic composite. The materials were systematically characterized using XRD, FTIR, nitrogen adsorption–desorption isotherms with BET analysis, SEM–EDX, and TEM to evaluate their crystallinity, functional groups, surface area and porosity, morphology, and elemental composition. The high surface area and microporous structure of UiO‐66‐NH 2 (900.67 m 2 /g) made it suitable for enzyme immobilization, whereas the Fe 3 O 4 @Cellu@UiO‐66‐NH 2 composite offered additional advantages such as magnetic recoverability and improved pore accessibility. Both materials were employed for cellulase immobilization via physical adsorption, yielding enzyme loading capacities of 290.63 mg/g for UiO‐66‐NH 2 and 295.47 mg/g for the composite. Reusability tests showed that more than 68% of enzymatic activity was retained after 10 cycles, whereas storage stability tests indicated over 52% activity retention after 30 days at 4°C. These findings underscore the superior stability, reusability, and immobilization efficiency of UiO‐66‐NH 2 ‐based composites, particularly Fe 3 O 4 @Cellu@UiO‐66‐NH 2 , making them promising candidates for industrial biocatalysis and enzyme reuse applications.