Triton CG‐110 as an alternative for cell lysis in manufacturing of adeno‐associated virus‐based gene therapy

Abstract

Triton X‐100 is one of the most widely used detergents for cell lysis in gene therapy product manufacturing. However, due to the aquatic toxicity of the degradation product, Triton X‐100 is regulated by the European Chemical Agency as a substance of very high concern. In this study, we aim to identify eco‐friendly detergent alternatives to replace Triton X‐100. Tween 20, Tween 80, n‐Dodecyl‐β‐D‐maltoside (DDM), and Triton CG‐110 were tested and compared with Triton X‐100. Triton CG‐110 demonstrated similar performance during the harvest and subsequent purification process for multiple AAV vectors at different scales. Therefore, Triton CG‐110 represents an effective and environmentally safe detergent alternative to Triton X‐100.