DOI: 10.7126/cumudj.1676547 ISSN: 1302-5805

Titanium-Platelet Rich Fibrin (T-PRF) versus Leucocyte Platelet Rich Fibrin (L-PRF): A Comparative evaluation of structural and elemental Characteristics using Inductively Coupled Plasma Optical Emission Spectrometry

Arigela Sai Vamsi, Aysha Jebin A, M.l. Prabhuji
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OBJECTIVES: In recent years there has been a growing interest in the use of platelet rich products for the treatment of many clinical conditions in dentistry. But there are possible chances of contamination of these products with silica. This study we aimed to define the structural characteristics and elemental analysis of platelet-rich product prepared in titanium tubes, Titanium-Platelet Rich Fibrin (T-PRF) and compare it with Leukocyte Platelet Rich Fibrin (L-PRF).METHODS: In this study blood is collected from the selected 10 patients through anti-cubital vein and used to detect the titanium and silica composition in T-PRF and L-PRF using Inductively Coupled Plasma – Optical Emission Spectrometry [ICP-OES]. The study also compared the structural difference between fibrin network in T-PRF and L-PRF using Phosphotungstic Acid Hematoxylin staining [PTAH] and hematoxylin and eosin staining method. RESULTS: On ICP-OES analysis there was a difference in the mean value of titanium ions and silica ions between the two groups. Almost 18.24 ppm of Titanium ions and 0.611ppm of silica ions were seen in the T-PRF group and 36.2 ppm of Silica ions and 3.56 ppm of titanium ions were seen in L-PRF group. On histological examination using haematoxylin and eosin and PTAH staining and focused under microscope there is significant structural difference in fibrin mesh formation and thickness was seen between T-PRF and L-PRF.This difference was statistically significant.CONCLUSION: This study is first of its kind analyzing the titanium and silica ions in L PRF and T-PRF using ICP -OES analysis, which will contribute to the improvement of L-PRF and its biocompatibility. This study emphasized that, T-PRF is having a strong fibrin network, with less potential contamination with silica. And it is safest regenerative material to use in clinical practice.

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