Tick Diversity Associated with the European Brown Hare (Lepus europaeus Pallas, 1778) in Croatia: Geographic Patterns and Species Composition

Ticks are hematophagous ectoparasites of major veterinary and public health importance, acting as vectors of numerous pathogens affecting wildlife, domestic animals, and humans. Data on tick species infesting lagomorphs in Croatia remain scarce. This study investigated the species composition and genetic diversity of ticks collected from European brown hares (Lepus europaeus) in Croatia during 2024–2025 using morphological and molecular methods. A total of 158 hares from 18 localities were examined, and ticks were detected on 145 individuals, resulting in an infestation prevalence of 91.8%. Morphological identification and molecular analyses revealed nine tick species. The tick community was dominated by Ixodes ricinus (59.1%; n = 821), followed by Ixodes festai (26.8%; n = 372) and Rhipicephalus bursa (9.6%; n = 133), together accounting for more than 95% of all collected ticks. Molecular analyses confirmed species identification and revealed 10 haplotypes of I. ricinus, eight of I. festai, two of I. ventalloi, and one in I. inopinatus. The results demonstrate that European brown hares are important hosts for diverse tick species in Croatia and provide baseline data for future studies on tick ecology, tick-borne pathogens, and wildlife health management.