The Significance of the Regulation Between tRF ‐31‐ U5YKFN8DYDZDD and BMPER in the Pathogenesis o

ABSTRACT

tsRNAs are a kind of small non‐coding single‐stranded RNA, which play an important role in many kinds of tumours. Previous studies have identified that tRF‐31‐U5YKFN8DYDZDD can be used as a novel tumour biomarker for the diagnosis and prognosis of gastric cancer (GC). In this study, we further explored the regulatory effect of tRF‐31‐U5YKFN8DYDZDD on tumour biological function in GC cells and related regulatory mechanisms. Inhibition of tRF‐31‐U5YKFN8DYDZDD can inhibit the proliferation, invasion, migration and angiogenesis of GC cells, while overexpression of tRF‐31‐U5YKFN8DYDZDD has the opposite effect. BMPER was shown to be a direct target of tRF‐31‐U5YKFN8DYDZDD in GC. Furthermore, the cytological effects of stable overexpression of BMPER were similar to the inhibition of tRF‐31‐U5YKFN8DYDZDD. And the attenuation of BMPER expression rescued the tRF‐mediated promotion of GC cells. WB showed that up‐regulation of tRF‐31‐U5YKFN8DYDZDD could increase the phosphorylation level of ERK and Smad1/5, and promote the expression of epithelial mesenchymal transition (EMT) and matrix metalloproteinases. Animal experiments in vivo show that down‐regulation of tRF‐31‐U5YKFN8DYDZDD can effectively inhibit tumour growth. These data suggest that tRF‐31‐U5YKFN8DYDZDD is a new tumour‐promoting factor and may be a potential new therapeutic target for GC.