DOI: 10.4103/mjbl.mjbl_734_23 ISSN: 1812-156X
The Effect of Toll-like Receptor 2 and IFN-γ Gene Polymorphism to Seropositive Toxoplasmosis in Recurrent Aborted Women
Mohammed Hassan Aubed, Ghada Basil Ali Alomashi Abstract
Background:
This study was carried out at the College of Medicine Al-Qadisiyah University in collaboration with the Women’s and Children’s in Wasit Hospital from the period of January to October 2022. It was done on one hundred two blood samples from aborted women and congenital anomalies and 50 blood samples from pregnant women as a control group.
Objective:
The objective was to determine the effect of toll-like receptor 2 (
TLR2
) and interferon-gamma (
INF-γ
) gene polymorphism on seropositive toxoplasmosis in aborted women.
Materials and Methods:
TLR2
and
INF-γ
gene polymorphism on aborted women were the focus of the study, which used allele primers, amplification refractory mutation system-polymerase chain reaction to detect the variations, and used to evaluate the genotypes single nucleotide polymorphisms (SNPs).
Results:
TLR2
gene polymorphism (rs4696480) and
IFN-γ
gene polymorphism (rs2430561) in Toxoplasma-aborted women revealed that homozygote mutant AA appeared in 20 and heterozygote mutant TA appeared in 53, both of which were risk factors for abortion in
TLR2
, whereas
IFN-γ
revealed that the homozygous mutant AA appeared at 24 and the heterozygote mutant TA appeared at 30, respectively, these risk factors for abortion were more prevalent in the patients group and the difference was not statistically significant (
P
= 0.072 and
P
= 0.338).
Conclusion:
According to the findings of the current study, IFN-
γ
SNP, which frequently connected with women who had abortions rather than those who were pregnant, and TLR-2 SNP, played a risk factor role in toxoplasma patients.