The Adjunctive Use of MSC Secretome for Guided Bone Regeneration: An Experimental In Vivo Investigation
Siddharth Shanbhag, Javier Sanz‐Esporrin, Javier Nuñez, Fernando Luengo, Manuel Toledano‐Osorio, Carina Kampleitner, Patrick Heimel, Barbara Schædl, F. Javier Vela Gonzalez, Samih Mohamed‐Ahmed, Cecilie Gjerde, Reinhard Gruber, Kamal Mustafa, Mariano SanzABSTRACT
Objective
To evaluate the efficacy of mesenchymal stromal cells' (MSC) secretome delivered as conditioned media (CM) as an adjunct to a xenograft (XG) and collagen membrane (MEM) for guided bone regeneration (GBR) in chronic non‐contained mandibular defects in minipigs.
Methods
Chronic bilateral mandibular defects were surgically created in seven minipigs. In a split‐mouth design, defects were treated with either CM‐functionalized (test) or native XG + MEM (control) and evaluated after 4 and 12 weeks. New bone formation was assessed using in vivo computed tomography (CT) and ex vivo micro‐CT, histology and immunohistochemistry (IHC). Quantitative CT, micro‐CT and histomorphometric data were statistically analysed.
Results
No significant differences in new bone formation were observed between the test and control group at either timepoint. These findings could be partly explained by a high intrinsic healing capacity and continual growth of the animals. Minimal residual XG material was detected in both groups at 4 and 12 weeks. When present, these remnants were associated with multinucleated Cathepsin K‐positive cells (IHC) with no observable differences between the groups.
Conclusion
The adjunctive use of