DOI: 10.1534/genetics.110.120717 ISSN:
Targeting DNA Double-Strand Breaks with TAL Effector Nucleases
Michelle Christian, Tomas Cermak, Erin L Doyle, Clarice Schmidt, Feng Zhang, Aaron Hummel, Adam J Bogdanove, Daniel F Voytas- Genetics
Abstract
Engineered nucleases that cleave specific DNA sequences in vivo are valuable reagents for targeted mutagenesis. Here we report a new class of sequence-specific nucleases created by fusing transcription activator-like effectors (TALEs) to the catalytic domain of the FokI endonuclease. Both native and custom TALE-nuclease fusions direct DNA double-strand breaks to specific, targeted sites.