DOI: 10.1126/science.1065810 ISSN:

Systematic Genetic Analysis with Ordered Arrays of Yeast Deletion Mutants

Amy Hin Yan Tong, Marie Evangelista, Ainslie B. Parsons, Hong Xu, Gary D. Bader, Nicholas Pagé, Mark Robinson, Sasan Raghibizadeh, Christopher W. V. Hogue, Howard Bussey, Brenda Andrews, Mike Tyers, Charles Boone
  • Multidisciplinary

In Saccharomyces cerevisiae , more than 80% of the ∼6200 predicted genes are nonessential, implying that the genome is buffered from the phenotypic consequences of genetic perturbation. To evaluate function, we developed a method for systematic construction of double mutants, termed synthetic genetic array (SGA) analysis, in which a query mutation is crossed to an array of ∼4700 deletion mutants. Inviable double-mutant meiotic progeny identify functional relationships between genes. SGA analysis of genes with roles in cytoskeletal organization ( BNI1 , ARP2 , ARC40 , BIM1 ), DNA synthesis and repair ( SGS1 , RAD27 ), or uncharacterized functions ( BBC1 , NBP2 ) generated a network of 291 interactions among 204 genes. Systematic application of this approach should produce a global map of gene function.

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