DOI: 10.1146/annurev.biochem.77.061906.092014 ISSN:

Super-Resolution Fluorescence Microscopy

Bo Huang, Mark Bates, Xiaowei Zhuang
  • Biochemistry

Achieving a spatial resolution that is not limited by the diffraction of light, recent developments of super-resolution fluorescence microscopy techniques allow the observation of many biological structures not resolvable in conventional fluorescence microscopy. New advances in these techniques now give them the ability to image three-dimensional (3D) structures, measure interactions by multicolor colocalization, and record dynamic processes in living cells at the nanometer scale. It is anticipated that super-resolution fluorescence microscopy will become a widely used tool for cell and tissue imaging to provide previously unobserved details of biological structures and processes.

More from our Archive