Suitability of a Fluorescence Method for Flavin Mononucleotide Determination in Modified HTK-Based Perfusion Solutions During Hypothermic Oxygenated Perfusion of Liver Grafts
Yulia B. Basok, Eugenia G. Kuznetsova, Aleksandra D. Belova, Dmitriy A. Morozov, Mikhail A. Boldyrev, Artem R. Monakhov, Nikita V. Grudinin, Vladimir K. Bogdanov, Denis M. Bondarenko, Stepan I. Zubenko, Nidzhat M. Yusuf, Sergey V. GautierA comprehensive assessment of allograft quality is essential for predicting successful transplantation and graft survival. Elevated levels of flavin mononucleotide (FMN) in perfusate obtained during hypothermic oxygenated perfusion (HOPE) may serve as a promising predictor of early graft dysfunction and post-transplant complications. The aim of this study was to evaluate the feasibility of applying an FMN determination method to HTK-based perfusion solutions used during liver machine perfusion. The spectrofluorimetric method for FMN determination in modified HTK perfusion solution (modHTK) was evaluated for the following parameters: linearity, accuracy, repeatability, reproducibility, and stability. The study demonstrated the suitability of the quantitative FMN determination method for modHTK solution used during HOPE of liver grafts.