SUBCHRONIC TOXICITY ASSESSMENT OF THE WOUND HEALING AGENT SPUMAZOLE IN THE FOAM AEROSOL FORM

The objective of the study is to comprehensively evaluate the subchronic toxicity of Spumazole to determine its safety for use in veterinary practice. The advantages and relevance of developing combination products for veterinary use are discussed, as well as the importance of providing them with rational dosage forms as a prerequisite for optimal pharmacotherapeutic action. A new potential combination product, Spumazole, based on a combination of natural and synthetic active ingredients with multimodal pharmacological properties for the treatment of complicated wounds in animals, is considered as an example. Spumazole is a combination product in an innovative foam aerosol form for the treatment of various types of wounds in animals, combining active ingredients of plant (terpenoids, polyphenolic compounds, hydroxycinnamic acids, etc.), biotechnological (proteolytic enzyme), and synthetic (quinoxaline derivative with a broad spectrum of antimicrobial action) origin, possessing antimicrobial, anti-inflammatory, necrolytic, antioxidant, and regenerative properties. The results of the study of the general toxic properties of Spumazole under subchronic experimental conditions are presented as a necessary stage of preclinical research. Subchronic toxicity parameters were studied in compliance with Russian standards and regulations for conducting scientific experiments on laboratory animals using generally accepted methods. They were used in four groups of white laboratory rats at the vivarium of the Krasnodar Scientific Center for Veterinary Medicine, taking into account previously obtained acute toxicity studies. Long-term observations of the laboratory animals tested with Spumazole revealed no signs of intoxication, dystrophic, inflammatory, or other pathological changes in internal organs.