Studies of FBJ Osteosarcoma Virus in Tissue Culture. I. Biologic Characteristics of the “C”-Type Viruses
Jay A. Levy, Janet W. Hartley, Wallace P. Rowe, Robert J. HuebnerSummary
The FBJ osteosarcoma virus (FBJ-MSV) is the only known naturally occurring murine sarcoma virus (MSV); it is unique among strains of MSV in producing primarily osteosarcomas in mice. A non-pathogenic virus, FBJ-MLV (murine leukemia virus), was found associated at high titer with the MSV. This MSV/MLV complex was similar to the RNA members of the murine oncogenic virus group, having typical “C”-type morphology, group-specific antigen, density of 1.16 g/cm3, and chloroform sensitivity, and may be classified in the N-tropic Gross-AKR subgroup. FBJ-MSV induced foci of cell alteration in rat and mouse tissue-culture cells which differed morphologically and temporally from those produced by other MSV's. The foci formed in mouse embryo cells had the unusual capacity to propagate independently.