DOI: 10.1099/ijsem.0.005805 ISSN:

Starkeya nomas sp. nov., a prosthecate and budding bacterium isolated from an immunocompromized patient

José Miguel Sahuquillo-Arce, Mariana Reyes-Prieto, Alicia Hernández-Cabezas, José Miguel Molina-Moreno, Juan Antonio Saez-Nieto, María del Pilar Marín, María Isabel Alcoriza-Balaguer, Agustín Lahoz, Jaime Sanz, Lola González-Tarancón, María Loreto Ferrús, Llúcia Martínez-Priego, Adolfo Magraner-Martínez, José Luis López-Hontangas
  • General Medicine
  • Ecology, Evolution, Behavior and Systematics
  • Microbiology

Strain HF14-78462T is an environmental bacterium found in clinical samples from an immunocompromized patient in 2014 at Hospital Universitari i Politècnic La Fe (Valencia, Spain). Phenotypically, strain HF14-78462T cells were Gram-stain-negative, aerobic, non-spore forming and non-motile small rods which formed mucous and whitish-translucent colonies when incubated at 20–36 °C. Phylogenetic analyses based on the 16S rRNA genes and the whole genomes of closest sequenced relatives confirmed that strain HF14-78462T is affiliated with the genus Starkeya . The strain was oxidase, catalase and urease positive; but indole, lysine decarboxylase, ornithine decarboxylase and DNase negative, did not produce H2S and was able to utilize a wide variety of carbon sources including acetamide, adonitol, amygdalin,

-arabinose, citric acid, glucose, mannitol and melibiose. Unlike Starkeya novella and Starkeya koreensis , strain HF14-78462T failed to grow in thiosulphate-oxidizing media and had a narrower temperature growth range. Its genome was characterized by a size of 4.83 Mbp and a C+G content of 67.75 mol%. Major fatty acids were C18:1 ω7c, cyclo C19 : 0 and C16 : 0, its polar acids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and an aminophospholipid; while the ubiquinones were Q9 (1.8 %) and Q10 (98.2 %). Digital DNA–DNA hybridization values were 41 and 41.4 against S. novella and S. koreensis , respectively, while average nucleotide identity values were around 84 %. Phenotypic, average nucleotide identity and phylogenomic comparative studies suggest that strain HF14-78462T is a new representative of the genus Starkeya and the name Starkeya nomas sp. nov. is proposed. The type strain is HF14-78462T (=CECT 30124T=LMG 31874T).

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