DOI: 10.1126/science.1089035 ISSN:

Spatiotemporal Rescue of Memory Dysfunction in Drosophila

Sean E. McGuire, Phuong T. Le, Alexander J. Osborn, Kunihiro Matsumoto, Ronald L. Davis
  • Multidisciplinary

We have developed a method for temporal and regional gene expression targeting (TARGET) in Drosophila and show the simultaneous spatial and temporal rescue of a memory defect. The transient expression of the rutabaga -encoded adenylyl cyclase in the mushroom bodies of the adult brain was necessary and sufficient to rescue the rutabaga memory deficit, which rules out a developmental brain defect in the etiology of this deficit and demonstrates an acute role for rutabaga in memory formation in these neurons. The TARGET system offers general utility in simultaneously addressing issues of when and where gene products are required.

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