DOI: 10.1126/science.aaa6090 ISSN:

Spatially resolved, highly multiplexed RNA profiling in single cells

Kok Hao Chen, Alistair N. Boettiger, Jeffrey R. Moffitt, Siyuan Wang, Xiaowei Zhuang
  • Multidisciplinary

Multiplexed RNA imaging in single cells

The basis of cellular function is where and when proteins are expressed and in what quantities. Single-molecule fluorescence in situ hybridization (smFISH) experiments quantify the copy number and location of mRNA molecules; however, the numbers of RNA species that can be simultaneously measured by smFISH has been limited. Using combinatorial labeling with error-robust encoding schemes, Chen et al. simultaneously imaged 100 to 1000 RNA species in a single cell. Such large-scale detection allows regulatory interactions to be analyzed at the transcriptome scale.

Science , this issue p. 10.1126/science.aaa6090

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