Solution and solid‐state ³³S NMR studies of ³³S‐labeled taurine

Abstract

Sulfur‐33(³³S) stable‐isotope labeled taurine, 2‐aminoethanesulfonic acid, has been synthesized, and a series of solution and solid‐state ³³S nuclear magnetic resonance (NMR) experiments at 14.1 and 18.8 T, respectively, have been carried out at room temperature. The single peak of a solution ³³S NMR spectrum in 0.1‐mM [³³S]‐taurine in D₂O can be observed with the signal‐to‐noise (S/N) ratio of 9 in 40,000 scans, which paves the way toward in vivo analysis of pharmacokinetics and metabolism of ³³S‐labeled taurine. Undistorted magic‐angle‐spinning (MAS) and static ³³S NMR spectra of polycrystalline [³³S]‐taurine are observed with sufficient S/N ratios for analysis, and the magnitudes of ³³S EFG and CS tensors can be obtained.