DOI: 10.1126/science.aaw1219 ISSN:
Slide-seq: A scalable technology for measuring genome-wide expression at high spatial resolution
Samuel G. Rodriques, Robert R. Stickels, Aleksandrina Goeva, Carly A. Martin, Evan Murray, Charles R. Vanderburg, Joshua Welch, Linlin M. Chen, Fei Chen, Evan Z. Macosko- Multidisciplinary
Gene expression at fine scale
Mapping gene expression at the single-cell level within tissues remains a technical challenge. Rodriques et al. developed a method called Slide-seq, whereby RNA was spatially resolved from tissue sections by transfer onto a surface covered with DNA-barcoded beads. Applying Slide-seq to regions of a mouse brain revealed spatial gene expression patterns in the Purkinje layer of the cerebellum and axes of variation across Purkinje cell compartments. The authors used this method to dissect the temporal evolution of cell type–specific responses in a mouse model of traumatic brain injury.
Science
, this issue p.