Sinisalibacter aestuarii sp. nov., isolated from estuarine sediment of the Arakawa River

A Gram-stain-negative, rod-shaped, non-motile and strictly aerobic bacterium, which showed biofilm-forming ability on polystyrene, designated as strain B-399^T, was isolated from the estuarine sediment of the Arakawa River near Tokyo Bay. It grew at pH 6.0–8.5, at 15–35 °C and in the presence of 0–7.5 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain B-399^T was clustered in the genus Sinisalibacter and has 96.94 % sequence similarity to Sinisalibacter lacisalsi X12M-4^T, which was the only validly described species in this genus. On the basis of our genome sequencing analyses, the average nucleotide identity and digital DNA–DNA hybridization values between strains B-399^T and S. lacisalsi X12M-4^T were 79.54 and 22.30 %, respectively, which confirms that strain B-399^T represents a novel species of the genus Sinisalibacter . The draft genome size and the DNA G+C content of strain B-399^T were 4.12 Mb and 65.2 mol%, respectively. The major fatty acids (>10 %) of strain B-399^T were C_16 : 0, summed feature 8 (C_18 : 1  ω6c and/or C_18 : 1  ω7c) and C_19 : 0 cyclo ω8c. The polar lipids were phosphatidylcholine, phosphatidylglycerol, an unidentified phospholipid, an unidentified aminolipid and unidentified lipids. The respiratory quinone was Q-10. These chemotaxonomic features were almost coincident with those of the genus Sinisalibacter . Therefore, strain B-399^T should be classified as representing a new species of the genus Sinisalibacter , for which the name Sinisalibacter aestuarii sp. nov. is proposed. The type strain is B-399^T (=NBRC 115629^T=DSM 114148^T).