Single-cell RNA-seq highlights intratumoral heterogeneity in primary glioblastomaAnoop P. Patel, Itay Tirosh, John J. Trombetta, Alex K. Shalek, Shawn M. Gillespie, Hiroaki Wakimoto, Daniel P. Cahill, Brian V. Nahed, William T. Curry, Robert L. Martuza, David N. Louis, Orit Rozenblatt-Rosen, Mario L. Suvà, Aviv Regev, Bradley E. Bernstein
Cancer at single-cell resolution
Single-cell sequencing can illuminate the genetic properties of brain cancers and reveal heterogeneity within a tumor. Patel et al. examined the genome sequence of single cells isolated from brain glioblastomas. The findings revealed shared chromosomal changes but also extensive transcription variation, including genes related to signaling, which represent potential therapeutic targets. The authors suggest that the variation in tumor cells reflects neural development and that such variation among cancer cells may prove to have clinical significance.
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