Simultaneous Estimation of Artemether and Lumefantrine in Tablet Formulation by UV-Visible Spectroscopy using Simultaneous Equation Method

In the present work, a simple, accurate and precise method has been developed and validated for the simultaneous estimation of antimalarial agents, Artemether & Lumefantrine and in their combined dosage form i.e. tablets by UV Spectrophotometric Method. It employs estimation of drugs by Simultaneous Equation Method (SEM) using 250 nm and 305 nm in Chloroform as λmax values of Artemether and Lumefantrine respectively. Both drugs obey Beer-Lamberts law in the concentration range of 4.5 μg/ml to 82.5 μg/ml. Validation of the proposed methods was carried out for its precision, linearity and limit of detection according to specifications. The recovery studies ascertained accuracy and reproducibility. The method was applied successfully for the estimation of Artemether and Lumefantrine in tablet dosage form without the interference of common excipients