DOI: 10.1002/jbm.b.70102 ISSN: 1552-4973
Silver and Gold Nanoparticles Promote Antimicrobial Activity and Modulate Inflammation in
Escherichia coli
‐Infected Wounds
Rubya Pereira Zaccaron, Rusilania Tozi Barbieri, Laura de Roch Casagrande, Igor Ramos Lima, Gabrielli Martins, Camila da Costa, Isabella Aguiar Cardoso, Ellen de Pieri, Luiz César Guarita‐Souza, Ricardo Andrez Machado‐De‐Ávila, Anand Thirupathi, Paulo Cesar Lock Silveira ABSTRACT
Silver and gold nanoparticles promote antimicrobial activity and modulate inflammation in
Escherichia coli
‐infected wounds. Seventy‐two Wistar rats were randomly assigned to six experimental groups (
n
= 12). Epithelial wounds were induced and contaminated with
E. coli
, followed by topical treatment for 8 days. Treatment with Cur‐Ag NPs, Cur‐Au NPs, and Cur‐Ag‐Au NPs significantly increased wound contraction compared to the contaminated wound group (
p
< 0.05). The Cur‐Ag‐Au NPs group showed a marked reduction in inflammatory infiltrate, accompanied by a significant decrease in IL‐1
β
levels (
p
< 0.05) and increased levels of IL‐10 and TGF‐
β
(
p
< 0.05). Oxidative stress parameters were significantly improved, with reduced nitrite levels (
p
< 0.05) and increased glutathione (GSH) levels (
p
< 0.05). Additionally, treated groups exhibited a significant reduction in
E. coli
colony‐forming units between days 3 and 7 (
p
< 0.05), indicating antimicrobial activity. These findings demonstrate that biosynthesized AgNPs and AuNPs exert significant antimicrobial, anti‐inflammatory, and antioxidant effects, contributing to enhanced tissue repair in infected wounds.