DOI: 10.1093/ofid/ofag720 ISSN: 2328-8957
Shotgun metagenomics versus 16S rDNA Sanger sequencing for the diagnosis of blood culture-negative endocarditis: a draw?
Safa Ben Brahim, Guilhem Royer, Hervé Jacquier, Raphaël Lepeule, François Goehringer, Justine Cheval, Christophe Rodriguez, Corentine Alauzet, Paul-Louis WoertherAbstract
Although valuable for diagnosing blood culture-negative infective endocarditis, 16S rDNA sequencing and metagenomics on valve/vegetation have never been compared using identical DNA extracts. We compared methods on surgical samples from definite, possible, and rejected infective endocarditis involving culture-negative native valves. Positivity rates were similar, taxonomic resolution being higher for metagenomics.