DOI: 10.1099/ijsem.0.006000 ISSN:

Shinella sedimenti sp. nov., isolated from sediment of Zhairuo Island located in the East China Sea

Gen Chen, Min He, Ke-Jing Li, Kai-Wen Zheng, Xing-Xing Tang, Sanjit Chandra Debnath, Pin-Mei Wang, Zhen Guo, Yi Hong, Dao-Qiong Zheng
  • General Medicine
  • Ecology, Evolution, Behavior and Systematics
  • Microbiology

A Gram-negative, rod-shaped and aerobic bacterial strain B3.7T, was isolated from the sediment of Zhairuo Island, Zhoushan city, Zhejiang Province, PR China. Maximum growth of strain B3.7T was observed at 30 °C when cultured in a medium containing 0.5 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences demonstrated that strain B3.7T belonged to the genus Shinella ; it showed the highest sequence similarity of 98.47 % to Shinella kummerowiae CCBAU 25048T. The average nucleotide identity and digital DNA–DNA hybridization values between strain B3.7T and its reference strains were 82.9–84.2 % and 26.1–27.3 %, respectively. Chemotaxonomic analysis indicated that the sole respiratory quinone was Q-10 and the predominant cellular fatty acids were C19 : 0 cyclo ω8c, C16 : 0, C18 : 1  ω7c 11-methyl and summed feature 8 (C18 : 1  ω7c and/or C18 : 1  ω6c). The polar lipid profile was composed of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, three unidentified phospholipids and two unidentified aminolipids. Collectively, strain B3.7T can be considered to represent a novel species, for which the name Shinella sedimenti sp. nov. is proposed. The type strain is B3.7T (=MCCC 1K07163T=LMG 32559T).

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