Screening of biomarkers related to lung adenocarcinoma based on construction of ceRNA regulation network
Chongyang Niu, Xiaoyu Meng, Chengbo Yuan, Xuelian Sun, Tan WangBackground:
Lung adenocarcinoma (LUAD) is a common malignant tumor with a poor prognosis and limited effective therapeutic targets. The underlying molecular regulatory mechanisms driving its progression remain largely unclear. The study objectives were to build a circRNA–miRNA–mRNA ceRNA regulation network of LUAD and to identify miRNAs and mRNAs significantly related to the prognosis .
Methods:
The gene expression data and GSE101684 were downloaded from the UCSC Xene and NCBI-GEO databases, respectively. The differentially expressed RNAs (DEcircRNAs, DEmiRNAs, and DEmRNAs; DERs) were obtained by the Limma package in R. Then, the differential LUAD-related genes were identified, and the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways of the differential LUAD-related genes were analyzed. Moreover, the circRNA–miRNA–mRNA ceRNA network of LUAD was built. The Kaplan–Meier (K–M) survival curve analysis of ceRNA network nodes was performed. In addition, the proliferation-related ceRNA network was built.
Results:
A total of 382 DEcircRNAs, 1907 DEmRNAs and 156 DEmiRNAs were acquired. A total of 245 differential LUAD-related genes were acquired, which were significantly associated with 189 GO biological processes (BP) and 17 KEGG pathways. Moreover, the ceRNA network of LUAD was built. The K–M survival curve analysis of ceRNA network nodes revealed that a total of 2 miRNAs (hsa-miR-96-5p and hsa-miR-125b-2-3p) and 22 mRNAs (CGNL1, CTHRC1, TK1, etc) were significantly related to the prognosis. mRNAs were significantly enriched in 92 GO BPs (such as cell division, cell adhesion) and 9 KEGG pathways (such as cell cycle, HTLV-1 infection). In addition, the proliferation-related ceRNA network was built.
Conclusion:
This research built a ceRNA regulation network of LUAD and is of great significance for identifying biomarkers related to the prognosis in LUAD.