Salivary oxidative stress biomarkers in thai adolescents and young adults with type 1 diabetes mellitus: A cross-sectional study
Thanwarat Aroonrangsee, Rachanin Chairatnathrongporn, Rudee Surarit, Kallapat Tansriratanawong, Jeerunda Santiprabhob, Chatkoew Boriboonhirunsarn, Ananya Promsudthi- General Dentistry
Abstract
Aims and Objectives:
The primary objectives of this study were to compare salivary oxidative stress (OS) biomarker levels in patients with type 1 diabetes mellitus (T1DM) and without T1DM (non-T1DM) and evaluate the relationships between diabetes, periodontal status, and OS biomarker levels.
Materials and Methods:
Twenty patients with T1DM and 20 age-matched patients without T1DM were enrolled. All participants were 15–23 years of age and had permanent dentition. Unstimulated whole saliva was collected in a sterile test tube before examination of clinical periodontal parameters, including bleeding on probing (BOP). Salivary levels of OS biomarkers—malondialdehyde, protein carbonyl, total oxidant status (TOS), and total antioxidant capacity—were determined using oxidative and antioxidative assays followed by spectrophotometric measurement at 375–532 nm. The relationships between diabetes, periodontal status, and OS biomarkers were analyzed using multiple linear regression.
Results:
TOS was significantly lower in the T1DM group compared with the non-T1DM group (5.06 ± 0.39 vs. 6.44 ± 0.51 µmol H2O2 Eq/l,
Conclusion:
Salivary TOS levels were related to both diabetes status and the extent of gingival inflammation. Further studies to elucidate the role of OS in relation of periodontal disease and T1DM are required.