RP-HPLC Method Development and Validation for the Etonogestrel and Ethinyl Estradiol in Pharmaceutical Dosage form

A simple, Accurate, precise method was developed for the simultaneous estimation of the Ethinylestradiol and Etonogestrel in pharmaceutical dosage form. Chromatogram was run through BDS C18 150 x 4.6mm, 5. Mobile phase containing Buffer 0.01N Na2HPO4: Acetonitrile taken in the ratio 70:30 was pumped through column at a flow rate of 1 ml/min. Buffer used in this method was NA2HPO4 Buffer. Temperature was maintained at 30°C. Optimized wavelength selected was 230nm. Retention time of Ethinylestradiol and Etonogestrel were found to be 2.163 min and 2.824. %RSD of the Ethinylestradiol and Etonogestrel were and found to be 0.8 and 0.5 respectively. %Recovery was obtained as 100.20% and 100.55% for Ethinylestradiol and Etonogestrel respectively. LOD, LOQ values obtained from regression equations of Ethinylestradiol and Etonogestrel were 0.03, 0.10 and 0.01, 0.02 respectively. Regression equation of Etonogestrel is y = 19522x + 504.57, and y = 27207x+358.27of Ethinylestradiol. Retention times were decreased and that run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.