DOI: 10.4103/mjbl.mjbl_656_23 ISSN: 1812-156X

Role of Human Herpes Virus-6 and Interleukin-17 Gene Polymorphism in Iraqi Patients with Acute Myleloid Leukemia

Worood Najah Abdulhadi, Shakir H. Mohammed Al-Alwany
Show PDF Cite

Abstract

Background:

Patients with lymphoproliferative diseases, such as lymphoma and leukemia, had peripheral blood leucocytes when the human herpes virus-6 (HHV-6) was initially discovered.

Objective:

Investigate the level of incidence of HHV-6-A and interleukin-17 (IL-17) gene polymorphism in Iraqi patients with acute myeloid leukemia (AML).

Materials and Methods:

100 freshly whole blood were obtained from patients with acute myeloid leukemia enrolled in this study; whereas control groups in current study included 100 freshly whole blood. Viral and total DNA genomic extraction were done to detect the HHV-6-A by PCR technique and IL-17 gene polymorphism by sequencing, respectively.

Results:

The positive of viral genome extraction was found in 42% (42/100) of the specimens which have viral genome, whereas 58% (58/100) specimens do not contain viral genome. The rate of human herpesvirus-6 infection according to the PCR was 38.1% (16/42) whereas the negative result was 61.9% (26/42). The results showed that DNA polymorphism distribution was according to GG, AA, and GA genotypes of the IL-17 rs2275913 polymorphism, which were 37.1%, 57.1%, and 5.7% in the patient with AML group, whereas in the control group, 71.4% (5/7 cases) and 28.6% (2/7 cases) were observed, respectively.

Conclusion:

In view of the relatively small numbers included in our study, the present results indicate the possibility that HHV-6 A as well as IL-17 rs2275913 may play a role in the tumor biology of the examined subset of acute myeloid leukemia and may contribute to their development.

More from our Archive