Response of Zostera japonica rhizosphere bacteria to ocean acidification

Amidst global climate change, the escalating atmospheric CO ₂ levels have intensified ocean acidification (OA), significantly impacting the structure and function of marine ecosystems. Seagrass beds, representative nearshore ecosystems, play a pivotal role in carbon sequestration, biodiversity preservation, and nearshore environmental equilibrium. Rhizosphere microorganisms within seagrass beds, essential components of the ecosystem, drive material cycling and energy flow. Their community structure and functions demonstrate heightened sensitivity to environmental variations. While previous studies have primarily focused on the effects of ocean acidification on seagrass hosts, limited attention has been given to the rhizosphere. Therefore, this study selected Zostera japonica as the focal species and systematically evaluated changes in the structure and function of the rhizosphere bacterial community across varying acidification levels (400 ppm, 1,000 ppm, 2,000 ppm CO ₂ ) within an ocean acidification context. The results revealed a significant decline in the richness and diversity of the rhizosphere bacterial community under acidification, accompanied by shifts in community composition characterized by an increase in the relative abundance of Bacteroidota and Tenacibaculum with escalating acidification levels. In high acidification conditions, bacterial network interactions exhibited a trend toward simplification; yet the number of key taxonomic units increases, and there was a shift in community assembly from stochastic to deterministic processes. Functional predictions indicated the enhancement of microbial carbon sequestration and nitrogen fixation under acidification, while denitrification and specific sulfur metabolism pathways were inhibited. This implies that in acidified environments, the rhizosphere bacterial community may enhance carbon and nitrogen fixation to uphold nutrient supply.

IMPORTANCE

Against the background of escalating global climate change and ocean acidification, seagrass beds, as crucial blue carbon sink ecosystems, face formidable challenges to their ecological functions and stability. Rhizosphere microorganisms of seagrasses, serving as the “second genome” of the seagrass host, play a central role in material cycling, nutrient supply, and system stability within seagrass beds. They are a key biological component that supports seagrass adaptation to environmental changes. Therefore, investigating the response and adaptation mechanisms of seagrass rhizosphere bacterial communities under ocean acidification is essential for deepening our understanding of the stability and resilience of seagrass bed ecosystems.