Registration of ‘ID21’, a spring barley recombinant inbred line population
Demeke B. Mewa, Margaret R. Krause, Scott P. Fisk, Sara Argo Price, Laura J. Helgerson, Ann M. Caspersen, Gongshe Hu, Kathy Esvelt Klos, Phil Bregitzer, Patrick M. Hayes, Dongying GaoAbstract
Developing genetic populations is a fundamental task in the molecular mapping of many important agronomic traits, especially those determined by multiple genes and environmental factors. In this study, barley ( Hordeum vulgare L. subsp. vulgare ) genotypes—a feed barley variety, ‘Golf’, developed in England and a malting barley variety, ‘95SR316A’, bred in the United States—were used to develop a recombinant inbred line (RIL) population named ‘ID21’ ( H. vulgare L. subsp. vulgare; Reg. no. MP‐5, NSL 558939 MAP). A total of 219 RILs were generated via the single seed descent method and genotyped using the barley 50K single nucleotide polymorphism array platform. We observed segregation of morphological traits, including leaf sheath and auricle colors, among the RILs, and detected a single quantitative trait locus (QTL) associated with both traits on barley chromosome 7H that explained 87% of the variance. Five markers were found within the QTL confidence interval spanning a 3‐cM genetic distance. Adult‐plant resistance to barley stripe rust was evaluated in the field, with >60% of the RILs showing resistance responses. Seed of the 219 RILs has been deposited in the USDA National Plant Germplasm System, and the genotyping data are also publicly available, providing valuable resources for mapping complex traits and improving barley genetics.