DOI: 10.62425/jlasp.1863362 ISSN: 2791-8645

Protective Impact of Chia Seed Oil on Autophagic and Ferroptotic Cell Death in Methotrexate-Induced Liver and Kidney Damage

Ali Güngör, Mustafa Özkaraca, Mansur Seymen Seğmenoğlu, Uğur Çayanoğlu
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Chia seed oil (CSO) essential fatty acids along with various phenolic compounds. Methotrexate (MTX), which targets rapidly dividing cancer cells and immune system cells, is used in various tumor formations but causes undesirable effects on the kidneys, liver, and various organs. In our study, we primarily investigated the effects of chia seed oil on autophagy and ferroptosis in liver and kidney damage. For this purpose, we formed 3 groups with 21 male Balb/c mice. The CONTROL group received nothing except standard feed and water. The MTX group received a single dose of 20 mg/kg methotrexate intraperitoneally on day 1. The MTX-CSO group received a single dose of 20 mg/kg methotrexate intraperitoneally on day 1, along with 4 ml/kg of chia seed oil administered via gavage. Chia seed oil treatment was continued for 7 days. On the 8th day, the mice were euthanized, and liver and kidney tissues were analyzed using histopathological, immunohistochemical, and double immunofluorescence staining methods. In conclusion, the severe histopathological findings observed in the MTX groups were alleviated in the MTX-CSO group. Immunohistochemical and dual immunofluorescence staining results show that while MTX activates autophagy and ferroptosis markers, CSO suppresses them, exhibiting a protective and regulatory effect. Our research is the first original study to investigate the effect of CSO on autophagic and ferroptotic cell death.

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